Coding

Part:BBa_K535003

Designed by: iGEM11_UNAM-Genomics_ Mexico   Group: iGEM11_UNAM-Genomics_Mexico   (2011-09-25)

FeOx -> Clostridium acetobutylicums ferredoxin

Ferredoxins are small soluble proteins that contain iron-sulfur clusters and that usually mediate electron transfer in a range of metabolic reactions.

Their iron-sulfur clusters contain iron and sulfur atoms organized, the iron atoms can change in the oxidation state (+2 or +3) and in that way accept or discharge electrons. Thus, ferredoxins act as electron transfer agents in biological redox reactions.

In this case we use this Clostridium acetobutylicum’s ferredoxin to transfer electrons to a linked hydrogenase (part HydA) from the same organism. PFOR (part PFOR) should oxidize pyruvate to acetyl-CoA and then reduce the ferrodoxin so it can transfer the electron.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 102


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Categories
Parameters
None